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Stem cell gene therapy: the risks of insertional mutagenesis and approaches to minimize genotoxicity
Chuanfeng Wu, Cynthia E. Dunbar
Frontiers of Medicine 2011, Volume 5, Issue 4, Pages 356-371 doi: 10.1007/s11684-011-0159-1
Keywords: gene therapy hematopoietic stem cells insertional mutagenesis genotoxicity induced pluripotent stem cell
Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B
Ru Zhang,Qiang Wang,Lin Zhang,Saijuan Chen
Frontiers of Medicine 2015, Volume 9, Issue 1, Pages 90-99 doi: 10.1007/s11684-015-0390-2
Gene therapy provides a potential cure for hemophilia B, and significant progress has been achievedin liver-directed gene transfer mediated by adeno-associated viral vectors.vectors and hFIX expression cassette may positively contribute to the ultimate success of hemophilia B genetherapy.therapy of hemophilia B.
Keywords: factor IX hemophilia B liver-specific regulatory elements hydrodynamic gene transfer
Gene therapy for hemophilia B mice with scAAV8-LP1-hFIX
Wei Lu,Qingzhang Zhou,Hao Yang,Hao Wang,Yexing Gu,Qi Shen,Jinglun Xue,Xiaoyan Dong,Jinzhong Chen
Frontiers of Medicine 2016, Volume 10, Issue 2, Pages 212-218 doi: 10.1007/s11684-016-0438-y
Keywords: hemophilia B AAV8 hFIX gene therapy
Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing
Yu Cao, Xiaoxuan Liu, Ling Peng
Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 4, Pages 663-675 doi: 10.1007/s11705-017-1623-5
Keywords: gene therapy RNAi therapeutics dendrimer nanovectors gene silencing
Investigation of gene therapy of denovirus in immune suppression
XIA Xi, WANG Beibei, CAO Li, CHEN Gang, WU Peng, LU Yunping, ZHOU Jianfeng, MA Ding
Frontiers of Medicine 2008, Volume 2, Issue 4, Pages 386-390 doi: 10.1007/s11684-008-0074-2
Keywords: different reversible inflammation immunohistochemistry reconstructed adenovirus ciclosporin
Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain
Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI
Frontiers of Medicine 2009, Volume 3, Issue 2, Pages 141-147 doi: 10.1007/s11684-009-0041-6
Keywords: Rab9 lentivirus gene therapy gene transfer
Zhao DING, Zhishui CHEN, Xilin CHEN, Ming CAI, Hui GUO, Nianqiao GONG
Frontiers of Medicine 2009, Volume 3, Issue 2, Pages 204-210 doi: 10.1007/s11684-009-0039-0
Keywords: anti-ERK2 renal transplantation epithelial mesenchymal transition chronic allograft nephropathy
Antitumor immunity of human SART3 gene vaccine against mouse tumor
HE Yu, YANG Shuhua, LIU Yong, LI Tao
Frontiers of Medicine 2008, Volume 2, Issue 1, Pages 51-57 doi: 10.1007/s11684-008-0010-5
Keywords: antitumor therapy occurrence implantation DNA vaccine SART3 DNA
NES1/KLK10 and hNIS gene therapy enhanced iodine-131 internal radiation in PC3 proliferation
Jiajia Hu, Wenbin Shen, Qian Qu, Xiaochun Fei, Ying Miao, Xinyun Huang, Jiajun Liu, Yingli Wu, Biao Li
Frontiers of Medicine 2019, Volume 13, Issue 6, Pages 646-657 doi: 10.1007/s11684-018-0643-y
Keywords: androgen-independent prostate cancer normal epithelial cell-specific 1/kallikrein 10 sodium/iodide symporter radiation therapy
Xudong YU MM, Zengwu SHAO MD, Liming XIONG MD, Weiwei XU MM, Hezhong WANG MM, Huifa XU MM,
Frontiers of Medicine 2009, Volume 3, Issue 4, Pages 415-420 doi: 10.1007/s11684-009-0072-z
Keywords: tissue inhibitor of metalloproteinase-3 intervertebral disc rabbit gene therapy
Genetic and clinical markers for predicting treatment responsiveness in rheumatoid arthritis
Xin Wu, Xiaobao Sheng, Rong Sheng, Hongjuan Lu, Huji Xu
Frontiers of Medicine 2019, Volume 13, Issue 4, Pages 411-419 doi: 10.1007/s11684-018-0659-3
Keywords: rheumatoid arthritis gene clinical markers therapy
Development of a magnetite-gene complex for gene transfection
Jian XIN BM, Ze-Feng XIA MD, Kai-Xiong TAO MD, Kai-Lin CAI PhD, Gao-Xiong HAN MD, Xiao-Ming SHUAI MD, Ji-Liang WANG MD, Han-Song DU MD, Guo-Bin WANG PhD, Yan LUO MM,
Frontiers of Medicine 2010, Volume 4, Issue 2, Pages 241-246 doi: 10.1007/s11684-010-0032-7
Keywords: nanoparticle magnetite gene therapy magnetofection
Yue Yu, Jie He
Frontiers of Medicine 2013, Volume 7, Issue 2, Pages 157-171 doi: 10.1007/s11684-013-0272-4
Keywords: non-small-cell lung cancer molecular typing individualized medicine molecular-targeted therapy gene expression
Frontiers of Medicine 2022, Volume 16, Issue 4, Pages 584-595 doi: 10.1007/s11684-021-0844-7
Keywords: hemophilia A adeno-associated virus (AAV) human/rat hybrid factor VIII gene therapy dual chain strategy
Yanlin Zhao,Xiao Zhong,Xiaohong Ou,Huawei Cai,Xiaoai Wu,Rui Huang
Frontiers of Medicine 2017, Volume 11, Issue 1, Pages 120-128 doi: 10.1007/s11684-017-0501-3
Norepinephrine transporter (NET) transfection leads to significant uptake of iodine-131-labeled metaiodobenzylguanidine (131I-MIBG) in non-neuroendocrine tumors. However, the use of 131I-MIBG is limited by its short retention time in target cells. To prolong the retention of 131I-MIBG in target cells, we infected hepatocarcinoma (HepG2) cells with Lentivirus-encoding human NET and vesicular monoamine transporter 2 (VMAT2) genes to obtain NET-expressing, NET-VMAT2-coexpressing, and negative-control cell lines. We evaluated the uptake and efflux of 131I-MIBG both in vitro and in vivo in mice bearing transfected tumors. NET-expressing and NET-VMAT2-coexpressing cells respectively showed 2.24 and 2.22 times higher 131I-MIBG uptake than controls. Two hours after removal of 131I-MIBG-containing medium, 25.4% efflux was observed in NET-VMAT2-coexpressing cells and 38.6% in NET-expressing cells. In vivo experiments were performed in nude mice bearing transfected tumors; results revealed that NET-VMAT2-coexpressing tumors had longer 131I-MIBG retention time than NET-expressing tumors. Meanwhile, NET-VMAT2-coexpressing and NET-expressing tumors displayed 0.54% and 0.19%, respectively, of the injected dose per gram of tissue 24 h after 131I-MIBG administration. Cotransfection of HepG2 cells with NET and VMAT2resulted in increased 131I-MIBG uptake and retention. However, the degree of increase was insufficient to be therapeutically effective in target cells.
Keywords: norepinephrine transporter vesicular monoamine transporter 2 -MIBG gene therapy lentivirus
Title Author Date Type Operation
Stem cell gene therapy: the risks of insertional mutagenesis and approaches to minimize genotoxicity
Chuanfeng Wu, Cynthia E. Dunbar
Journal Article
Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B
Ru Zhang,Qiang Wang,Lin Zhang,Saijuan Chen
Journal Article
Gene therapy for hemophilia B mice with scAAV8-LP1-hFIX
Wei Lu,Qingzhang Zhou,Hao Yang,Hao Wang,Yexing Gu,Qi Shen,Jinglun Xue,Xiaoyan Dong,Jinzhong Chen
Journal Article
Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing
Yu Cao, Xiaoxuan Liu, Ling Peng
Journal Article
Investigation of gene therapy of denovirus in immune suppression
XIA Xi, WANG Beibei, CAO Li, CHEN Gang, WU Peng, LU Yunping, ZHOU Jianfeng, MA Ding
Journal Article
Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain
Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI
Journal Article
Adenovirus-mediated antisense ERK2 gene therapy ameliorates chronic allograft nephropathy in a rat model
Zhao DING, Zhishui CHEN, Xilin CHEN, Ming CAI, Hui GUO, Nianqiao GONG
Journal Article
Antitumor immunity of human SART3 gene vaccine against mouse tumor
HE Yu, YANG Shuhua, LIU Yong, LI Tao
Journal Article
NES1/KLK10 and hNIS gene therapy enhanced iodine-131 internal radiation in PC3 proliferation
Jiajia Hu, Wenbin Shen, Qian Qu, Xiaochun Fei, Ying Miao, Xinyun Huang, Jiajun Liu, Yingli Wu, Biao Li
Journal Article
Adenovirus-mediated tissue inhibitor of metalloproteinase-3 gene transfection inhibits rabbit intervertebral
Xudong YU MM, Zengwu SHAO MD, Liming XIONG MD, Weiwei XU MM, Hezhong WANG MM, Huifa XU MM,
Journal Article
Genetic and clinical markers for predicting treatment responsiveness in rheumatoid arthritis
Xin Wu, Xiaobao Sheng, Rong Sheng, Hongjuan Lu, Huji Xu
Journal Article
Development of a magnetite-gene complex for gene transfection
Jian XIN BM, Ze-Feng XIA MD, Kai-Xiong TAO MD, Kai-Lin CAI PhD, Gao-Xiong HAN MD, Xiao-Ming SHUAI MD, Ji-Liang WANG MD, Han-Song DU MD, Guo-Bin WANG PhD, Yan LUO MM,
Journal Article
Molecular classification of non-small-cell lung cancer: diagnosis, individualized treatment, and prognosis
Yue Yu, Jie He
Journal Article
Long-term correction of hemorrhagic diathesis in hemophilia A mice by an AAV-delivered hybrid FVIII composed of the human heavy chain and the rat light chain
Journal Article